Journal: eNeuro

Article Title: FKBP51 in the Oval Bed Nucleus of the Stria Terminalis Regulates Anxiety-Like Behavior

doi: 10.1523/ENEURO.0425-21.2021

Figure Lengend Snippet: Fkbp5+ cells in the ovBNST coexpress with the neuropeptides Crh and Tac2 and their number is significantly increased after exposure to ASR. A , Fkbp5 and Tac2 are coexpressed in the ovBNST, as can be seen in detail (violet outline arrow). Fkbp5 and Crh are also coexpressed in the ovBNST as shown in detail (violet arrow). B , Expression patterns of Fkbp5 with Tac2 and Crh in the ovBNST also strongly overlapped (gray outline arrow). In addition, there were some cells that expressed Fkbp5 only (gray arrow). C , Quantification of the number of cells expressing Fkbp5 only, coexpressing Fkbp5 and Tac2 , coexpressing Fkbp5 and Crh , and coexpressing Fkbp5 , Tac2 , and Crh after exposure to ASR resulted in significant upregulation across all cell types. Scale bar ( A , B ): 25 μm. Data are mean ± SEM; * p < 0.05, ** p < 0.01.

Article Snippet: Manipulation of FKBP51 was performed using adeno-associated bicistronic AAV1/2 vectors (AAV1/2-HA-CAG-FKBP5-1 and AAV1/2-CAG-null, GeneDetect; AAV1-CMV-Cre and AAV1/2-CAG-empty-IRES-EGFP, Addgene; AAV1/2-ESARE-ER T2 CreER T2 ).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Molecular studies into cell biological role of Copine-4 in Retinal Ganglion Cells

doi: 10.1371/journal.pone.0255860

Figure Lengend Snippet: (A, B) Intraocular injections of AAV1-Cpne4 viruses were done in P0 or P14 Brn3b Cre/WT mice eyes. Blebs (indicated by boxes and arrows ) were seen occasionally on dendrites of Cpne4 infected RGCs. For controls, AAV1-eGFP was similarly injected in P0 Brn3b Cre/WT mice (C, D). No blebs were observed in the control infected RGCs. Airy scan images for one of the blebs (A’) and adjoining dendrites (A”). (E) Measurement of the ‘bleb’ areas for Cpne4 infected RGCs as compared to regular varicosities on controls shows a significant difference between them while no difference between P0 and P15 Cpne4 infected retinas. (F, G) Areas and lamination measurements indicate no differences between the Cpne4 transfected RGCs as compared to control transfected RGCs. (ID and OD: Inner and outer distance- distance between innermost or outermost tip of RGC dendrites and the INL; IPL: Width of the IPL). Scale bar for A-C: 50μm; Scale bar for A’, A”: 5μm.***, p< 0.001.

Article Snippet: Intraocular injections of 0.5 ìl (1e9 viral particles/ìl) of AAV1-Cpne4 or AAV1-eGFP control virus were done in the eyes using pulled glass capillaries fitted onto a Femtojet device (Eppendorf, Enfield, CT), as previously described [ ].

Techniques: Infection, Injection, Transfection